The high activity of the phenolic components may be further explained in terms of the alkyl substitution into the phenol nucleus, which is known to enhance the antimicrobial activity of. The antimicrobial activity of plant extracts and phytochemicals was evaluated with antibiotic susceptible and resistant microorganisms. Five plant species were investigated to evaluate their antibacterial activity against food poisoning bacteria including two strains of gram positive bacteria b. Research article open access antibacterial activities of selected edible plants extracts against multidrugresistant gramnegative bacteria doriane e djeussi1, jaures ak noumedem1, jackson a seukep1, aime g fankam1, igor k voukeng1, simplice b tankeo1, antoine hl nkuete2 and victor kuete1 abstract. All in all, data about specific antibiotic properties of coumarins are scarce, although many reports give. Antimicrobial activity of medicinal plant extracts and their synergistic effect on some selected pathogens desalegn amenu college of natural and computational science, biology. Antibacterial activity of plants extracts against pathogenic bacteria. For example the crude methanolic extracts of neem plant have been shown to have strong. Antimicrobial activity of medicinal plants against human. The residue the mixture was transferred into a percolator with overnight maceration. They are known to possess antioxidant, antiinflammatory, antibacterial, immunomodulatory and antisickling activities. The antibacterial activities of extracts were evaluated using the disk diffusion method as well as well diffusion method.
Apr 14, 2019 the plant extract treated fabrics were subjected to the antimicrobial activity test against the ecoli and s. The search for new antimicrobial natural products from plant materials is essential in order to curb the menace of multiple antibiotics resistant pathogens. Antimicrobial activity study of ethanolic extract of. The mbc was not determined for water extract since the antibacterial activity was low. Various parts of the plant such as the leaves, roots. Studies on some plant extracts for their antimicrobial. Data on antimicrobial activity measure as a zone of inhibition of aqueous extracts of eleven plant species and two antibiotics on. Evaluation of antibacterial activity of some medicinal.
Evaluation of antimicrobial activity of some plant extracts. Moreover, antimicrobial activity of different natural substances such as medicinal plant extract have been investigated against food borne bacteria. The antimicrobial effect of ethanol extracts of five plant extract against human bacteria namely streptococcus pyogenes atcc 19615, streptococcus. Antibioticresistant microorganisms have been an evergrowing concern over the past years. Box, 395, ethiopia abstract antibiotics provide the main basis for the therapy of microbial bacterial and fungal infections. Antimicrobial activity in leaf extract of neem azadirachta indica against human pathogenic bacteria. The antimicrobial activity from ethanol and petroleum ether extracts against multiple microorganisms was re ported 8. Antimicrobial activity of medicinal plant extracts and their synergistic effect on some selected pathogens desalegn amenu college of natural and computational science, biology department, wollega university, p. Azadirachta indica leaves and barks were collected from.
The fact that a plant extract exhibits antimicrobial activity is of interest, but this preliminary part of data should be trustworthy and allow researchers to compare results, avoiding work in which researchers use the antimicrobial activity investigation only as a complement to a phytochemical study. Methods for in vitro evaluating antimicrobial activity. Furthermore, chloroform and acetone extract from the leaves. Studies of antimicrobial properties of different leaf extract. Klebsiella the best antimicrobial activity was observed with eucalyptus leaf ethanol extracts. Determination of antimicrobial activity the agar well diffusion method described by pelczar et al. Determination of in vitro antimicrobial activity of five. Antimicrobial activity of azadirachta indica neem against. Some promising plants having antimicrobial activity against multidrug resistant strains. The extract of medicinal plants were prepared using soxhlet apparatus for alcoholic extract, and water reflux for aqueous extracts. Introduction nature has been a source of medicinal agents since ancient times.
The dried extract was kept at 4 c for antibacterial activity assay. Antimicrobial activity and phytochemical analysis of. Antibacterial and antifungal activities from leaf extracts. Pdf antibacterial activity of medicinal plant extracts.
Thus, a varied range of natural products of plant, fungi and lichen origin were tested against two bacterial species, staphylococcus aureus atcc 25923 and escherichia coli atcc 25922, by two variants of the agar diffusion. Antimicrobial activities of alcoholic extracts of neem leaves were used. The antimicrobial activity of plant extracts and phytochemicals was evaluated with antibiotic. Antimicrobial activity of crude extracts of three medicinal. Our results revealed that chloroform, butanol, ethyl acetate extract of a. The plant extract treated fabrics were subjected to the antimicrobial activity test against the ecoli and s.
Pdf antibacterial activity of medicinal plant extracts researchgate. Varying concentration of each extracts 200mgml, 150 mgml, 100mgml. Antimicrobial activity of polyphenols and alkaloids in middle. The fact that a plant extract exhibits antimicrobial activity is of interest, but this preliminary part of data should be trustworthy and allow researchers to compare results, avoiding work in which. Organic solvent extraction ethanol and methanol extracts. Introduction even though pharmacological industries have produced a number of new antibiotics in the last three decades, resistance to these drugs by microorganisms has increased. The antibacterial effect of some medicinal plant extracts.
Significant antimicrobial activity of plant extract had been observed. Absence of antimicrobial activity does not mean that the bioactive compounds are not present in the plant or the plant has no activity against microorganisms. In vitro antimicrobial activity of some medicinal plants. Antimicrobial activity of some medicinal plant extracts against bacteria causing diarrhoea by naomi tope komolafe submitted in accordance with the requirements for the degree of master of science in the subject life sciences at the university of south africa supervisor. Evaluation of antibacterial activity of some medicinal plants extracts. A method frequently used to screen plant extracts for antimicrobial activity is the agar disc diffusion technique morris et al. An inoculum suspension was swabbed uniformly to solidified 20 ml muellerhinton agar mha for bacteria and sabouraud dextrose agar sda for fungi and the. Antibacterial and antifungal activities from leaf extracts of. Collection of plant material and extract preparation. Results and discussion antibacterial activity all three extracts of the plant tested showed varying d eg rof an t ib c lv sh species table 1. The extract of three medicinal plants used in nigerian folk medicine showed a highly antibacterial activity against some food borne pathogens. Secondly, the method used to assess antimicrobial activity, and the choice of test organisms, varies between publications janssen et al. Antimicrobial activity and phytochemical analysis of calotropis gigantea root, latex extracts. Medicinal plants constitute an effective source of antimicrobial natural products.
Presence of inadequate quantities of active constituent or constituents in the extract to exhibit the antimicrobial activity can be the reason for the negative results. Extraction of plant latex is a complex emulsion of polymer micro particles in an aqueous medium. Antimicrobial activity of selected medicinal plants. Antimicrobial activity of fruits of aronia melanocarpa and sorbus aucuparia 197 table 2. Pdf antimicrobial activity of medicinal plant leaf. The extractions from latex are quit tricky in context with preparation of latex for the extraction purpose. May 15, 2019 antibioticresistant microorganisms have been an evergrowing concern over the past years. Aloe vera gel is a bactericidal agent causing against pseudomonas aeruginosa and acemannan and cite this article. Bacteria, antimicrobial activity, plant extracts, solvents. Bioactive aqueous extracts of twelve yucatecan plants. Materials and methods plates were prepared for the antibacterial activity. Antimicrobial activity of crude extracts of some plant leaves. Antimicrobial activity and phytochemical screening of stem.
Antibacterial and antifungal activities of plant part extracts against four pathogenic bacteria two grampositive and negative and three pathogenic fungi were investigated by the agar disk. However, the antimicrobial activity of several extracts of different plants was reported. Plant nametype of extract susceptible microorganism reference garcinia mangostana alpha. Antimicrobial activity of rosemary extracts rosmarinus. Antimicrobial activity of polyphenols and alkaloids in. Pdf antibacterial activity of some plant extracts researchgate. The search for new antimicrobial natural products from plant materials is. Acetone technical grade, merck was used as an extractant in the assays using a ratio of 1. Control experiments comprising inoculums without plant extract. Antibacterial activity of medicinal plant extracts article pdf available in brazilian journal of microbiology 384.
The significant activity shown by the extract of o. The antibacterial activity of extracts of nine plant species with good. The methanolic extract of caryophyllus aromaticus presented the highest antis. This has led researchers to direct their attention onto plants to be able to discover. Extraction of plant latex is a complex emulsion of polymer micro particles in an aqueous. The aim of this work was to evaluate the antimicrobial activity of synthesized heterocyclic compound shcs like as furan derivatefd 1furan2ylthioxopropenon, thiophene derivate thd 2cyano2scthiophene2yl1,2dithiol3ylidene. Research article open access antibacterial activities of. Antimicrobial activity of extracts from fruits of aronia. Antimicrobial, antioxidant activity and phytochemical. Antimicrobial activity of rosemary extracts rosmarinus officinalis l.
Thus, a varied range of natural products of plant, fungi and lichen origin were tested. Evaluating antimicrobial activity of aloe vera plant extract. The introduction of alkylation has been proposed to alter the distribution ratio between the aqueous and the nonaqueous phases. Moringa oleifera is a tropical tree whose many economic applications and facility of propagation are arousing growing international interest. Some bacteria showing resistance to certain antibiotics were sensitive. Phytochemical analysis of traditional medicinal plants and. It is in view of this, that the present research was set up to evaluate the antimicrobial activity of, using different.
The plant extracts showed antibacterial activity but not antifungal activity against any of the fungi used. Essential oils extracted from five fiber varie ties of. The total glycosides, cardenolide glycosides, anthraquinone glycosides isolated from. Antimicrobial tests for the leaves stem and roots of the studied plants were carried out against the pure cultures of and escherichia coli. Antibacterial activity of aqueous and ethanolic extracts of fresh and treated fruits, and fresh and treated extracts, expressed as inhibition zone diameter in millimeters. Dec 25, 2001 secondly, the method used to assess antimicrobial activity, and the choice of test organisms, varies between publications janssen et al. Antimicrobial activity of essential oils and other plant. Evaluation of antimicrobial activity of different solvent. Furthermore, chloroform and acetone extract from the leaves of anthocephalous cadamba had been reported to have prominent antimicrobial activity against several gram positive and gram negative human pathogenic bacteria 30. Evaluation of antibacterial activity of these plant extracts was recorded in table 2 and illustrated in fig. All in all, data about specific antibiotic properties of coumarins are scarce, although many reports give reason to believe that some utility may reside in these phytochemicals 26, 83, 193.
Determination of in vitro antimicrobial activity of five sri. Antibacterial and antifungal activities of plant part extracts against four pathogenic bacteria two grampositive and negative and three pathogenic fungi were investigated by the agar disk diffusion method. Pdf antimicrobial activity of medicinal plant leaf extracts. Antimicrobial activity of methanolic extract the antimicrobial activity of methanolic extract using disc diffusion method, p. Evaluation of antimicrobial activity of some plant. Antimicrobial activity of medicinal plant extracts and. For example the crude methanolic extracts of neem plant have been shown to have strong antibacterial activity8. The antimicrobial activity of the test organisms to the 11 plant extracts was screened by using the agarwell diffusion method perez et al. Water extract of garlic and clove possesses antimicrobial activity. Studies of antimicrobial properties of different leaf. Antioxidant, antihypertensive, antihyperglycemic, and antimicrobial. The middle east encompasses a wide spectrum of plant diversity with over 20,000 different species in habitats ranging from deserts to snowcapped mountains.
Exactly 200 mg of each dried mass of leaf was added to 2 ml of solvents aqueous, chloroform and ethanol. Antimicrobial activity of selected medicinal plants against. Pseudomonas showed resistance to all the solvents except to tulsi leaf ethanol extracts. Antimicrobial finish on textiles using plant extracts. Introduction medicinal plants are abundant source of antimicrobial molecules.
Antimicrobial activity of medicinal plants against human pathogenic bacteria 657 inflammatory properties of plants mahesh and satish, 2008. Antibacterial activity of plant extracts 247 brazilian journal of microbiology 2000 31. Evaluating antimicrobial activity of aloe vera plant. Medicinal plants, traditional uses, phytochemical effects, antimicrobial activity. In addition, the possible synergistic effects when associated with antibiotics were. The study has shown the presence of steroid, alkaloids and tannins. Antimicrobial activity of some plant extracts against bacterial strains.
However, ali mirzaei 29 showed that ethanolic extract of plant origanum vulgare shows significant antimicrobial activity. Effect of plant extract on bacterial culture bacterial culture extra negative control. Chemical composition, antifungal and anti aflatoxigenic activities of oils of ocimum species were investigated by some workers 21, 22. This has led researchers to direct their attention onto plants to be able to discover new possible antimicrobial compounds. Antibacterial activity of five medicinal plant extracts. Phytochemical and antibacterial activities of newbouldia.
The oil of the seeds, petroleum ether and methanol extracts of the whole plant of cannabis sativa belonging to the family cannabinaceae were screened for their antimicrobial activity against. Antimicrobial activity of some medicinal plant extracts against bacteria causing diarrhoea by naomi tope komolafe submitted in accordance with the. The antimicrobial activities of methanolic extracts of. General antimicrobial activity was documented in woodruff galium odoratum extracts. Antimicrobial, antioxidant activity ijpcr, june 2016, volume 8, issue 6 page 586 table 2. Antimicrobial activity of some plant extracts against. Nutrient agar 20ml was dispensed into sterile universal bottles. Aureus indicating different zones of inhibition table 1.